miracloth (Merck & Co)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Merck & Co miracloth
Miracloth, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miracloth/miracloth/pm41864205-644-6-7
Average 86 stars, based on 1 article reviews

miracloth - by Bioz Stars, 2026-10

86/100 stars

Images

Sonication:

Article Title: A circadian rheostat drives proton electrochemical gradients to optimize cell-type-specific growth in Arabidopsis.
Article Snippet: Article

Starch:

Article Title: Wheat mutants lacking Starch Synthase 1 have altered starch composition and cell wall content
Article Snippet: .. Starch was purified from 2.5-g samples of wholemeal flour by grinding in excess ice-cold water in a mortar then filtering through one layer of Miracloth (Merck, Haverhill, UK; www.merckgroup.com/uk-en ) into a 50-ml tube. ..

Purification:

Centrifugation:

Article Title: The unconventional G-protein AtYchF1 interacts with the ribosomal protein AtRPS7 to modulate selective translation for balancing plant growth and stress response in Arabidopsis.
Article Snippet: 649 Three hundred milligrams of plant powder were resuspended in 600 μL of polysome 650 extraction buffer [200 mM Tris HCl (pH 8), 50 mM KCl, 25 mM MgCl2, 1 mM DTT, 1 651 mM PMSF, 0.25% (v/v) Brij-35 (Cat#20150, Thermo Fisher Scientific, Waltham, MA, 652 USA), 0.25% (v/v) Igepal CA 630 (Cat#I8896, Merck, Rahway, NJ, USA), 0.25% (v/v) 653 Triton X-100 (Cat#X100, Merck, Rahway, NJ, USA), 0.25% (v/v) Tween 20 654 (Cat#P1379, Merck, Rahway, NJ, USA), 1% (v/v) polyoxyethylene (10) tridecyl ether, 655 1% (v/v) sodium deoxycholate, 100 μg mL-1 cycloheximide, 100 μg mL-1 656 chloramphenicol, 40 U mL-1 RNasin Ribonuclease Inhibitor (Cat#N2515, Promega, 657 Madison, WI, USA) and 1X HaltTM Protease Inhibitor Cocktail (Cat#78430, Thermo 658 Fisher Scientific, Waltham, MA, USA)], and centrifuged at 3,000 × g for 5 min at 4°C. .. 659 Then the supernatant was filtered through double-layered Miracloth (Cat#475855, 660 Merck, Rahway, NJ, USA), followed by centrifugation at 20,000 × g at 4°C for 10 min 661 to remove cell debris. .. Ten OD260 units of the clear polysome extract were loaded onto 662 a precooled 10-50% sucrose gradient containing 40 mM Tris-HCl (pH 8.4), 20 mM KCl, 663 10 mM MgCl2, 5 μg mL-1 cycloheximide, and 5 μg mL-1 chloramphenicol, prepared by 664 Gradient Master (BioComp Instruments, Fredericton, NB, Canada) in Ultra-Clear 665 centrifuge tubes (Cat#344059, Beckman Coulter, Brea, CA, USA) according to the 666 manufacturer’s instructions.

Review